Improved gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plans

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  • NAKAGAWA Tsuyoshi
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • SUZUKI Takamasa
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • MURATA Satoko
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • NAKAMURA Shinya
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • HINO Takeshi
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • MAEO Kenichiro
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • TABATA Ryo
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • KAWAI Tsutae
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • TANAKA Katsunori
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • NIWA Yasuo
    Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
  • WATANABE Yuichiro
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
  • NAKAMURA Kenzo
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • KIMURA Tetsuya
    Department of Sustainable Resource Science, Faculty of Bioresources, Mie University
  • ISHIGURO Sumie
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University

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タイトル別名
  • Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants

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We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.

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