Protection of<i>psbAII</i>Transcript from Ribonuclease Degradation<i>in Vitro</i>by DnaK2 and DnaJ2 Chaperones of the Cyanobacterium<i>Synechococcus elongatus</i>PCC 7942
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- WATANABE Satoru
- Department of Bioscience, Tokyo University of Agriculture
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- SATO Masumi
- Department of Bioscience, Tokyo University of Agriculture
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- NIMURA-MATSUNE Kaori
- Department of Bioscience, Tokyo University of Agriculture
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- CHIBAZAKURA Taku
- Department of Bioscience, Tokyo University of Agriculture
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- YOSHIKAWA Hirofumi
- Department of Bioscience, Tokyo University of Agriculture
書誌事項
- タイトル別名
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- Protection of psbAII Transcript from Ribonuclease Degradation in Vitro by DnaK2 and DnaJ2 Chaperones of the Cyanobacterium Synechococcus elongatus PCC 7942
- 公開日
- 2007
- DOI
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- 10.1271/bbb.60647
- 公開者
- 公益社団法人 日本農芸化学会
この論文をさがす
説明
Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Synechococcus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATP-dependent manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (1), 279-282, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206480353152
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- NII論文ID
- 10027510818
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8620049
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- PubMed
- 17213638
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- 本文言語コード
- en
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