FAB CID-MS/MS of In-Source Fragment Ions as a Means to Differentiate Linkage Isomers of Branched Oligosaccharides: Lea and Lex Type Glycoconjugates.

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  • OHASHI Yoko
    Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research
  • II Tadashi
    Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research
  • KUBOTA Masayuki
    Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research Department of Applied Physics and Chemistry, The University of Electro-Communications
  • NUNOMURA Shigeki
    Tokyo Pharmaceutical Research Laboratories, Nissin Food Products Co., Ltd.
  • NIWA Haruki
    Department of Applied Physics and Chemistry, The University of Electro-Communications
  • OHASHI Mamoru
    Department of Applied Physics and Chemistry, The University of Electro-Communications
  • OGAWA Tomoya
    Laboratory of Synthetic Cellular Chemistry, The Institute of Physical and Chemical Research Faculty of Agriculture, University of Tokyo
  • NAGAI Yoshitaka
    Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research Mitsubishikasei Institute of Life Sciences

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タイトル別名
  • FAB CID-MS MS of In-Source Fragment Ion

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Here we report that FAB CID-MS/MS, choosing an ion originating from the in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, is an excellent means to differentiate linkage isomers. FAB CID-MS/MS performed in this way results in a product ion spectrum, which is enriched with the fragmentation information, and which may thus indicate the position of the newly formed OH group already produced in the ion source. Linkage knowledge may be classified into one of three categories as described later. This method was applied to acidic Lewis-type glycoconjugates —putative ligands for selectins [L. L. W. Cooling, D.-S. Zhang, and T. A. W. Koerner, Trends in Glycosci. Glycotech., 9, 191 (1997)]— and their neutral analogs to differentiate linkage isomers by mass spectrometry. With these glycoconjugates FAB CID-MS/MS having [M+H]+s as the precursor ions is a good means to distinguish the linkage isomers only in simple cases, but not in general cases. By using ions originating from in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, we were able to differentiate between Lea and Lex.

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  • 質量分析

    質量分析 46 (1), 45-52, 1998

    一般社団法人 日本質量分析学会

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