書誌事項
- タイトル別名
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- Utility of Application of Liquid Chromatography/Triple Stage Mass Spectrometry (LC/MS3) for Proteome Analysis
- プロテオーム解析における液体クロマトグラフィー3段階質量分析法(LC/MS[3]) の有用性について
- プロテオーム カイセキ ニ オケル エキタイ クロマトグラフィー 3 ダンカイ シツリョウ ブンセキホウ LC MS3 ノ ユウヨウセイ ニ ツイテ
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抄録
Protein identification via mass spectrometry (MS) relies primarily on protein sequence databases. Identification resulting from a sequence database search in the tandem mass spectrometry (MS/MS) experiments for the digested peptides is derived from an algorithm based on statistical confidence, and therefore, it often pro- ides false-positive identifications. Although one can apply high-resolution MS such as Fourier-transform mass spectrometer (FT-MS) to obtain results with high confidence, the instrumentation is expensive and the results are still probability based. Therefore, we introduced data-dependent triple stage mass spectrometry (MS3) into our well-established liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis of linear ion trap MS to achieve evidence-based protein identification. Peptide sequencing by MS3 analysis can provide a direct evidence for a sequence elucidated by MS/MS data and help determine whether the search results are correct.
収録刊行物
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- 質量分析
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質量分析 57 (4), 317-325, 2009
一般社団法人 日本質量分析学会
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詳細情報 詳細情報について
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- CRID
- 1390001206495166848
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- NII論文ID
- 10025571784
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- NII書誌ID
- AN0010555X
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL書誌ID
- 10324162
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可