書誌事項
- タイトル別名
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- Molecular Characterization of Water Soluble Polymers by Field-Flow Fractionation Multiangle Light Scattering FFF-MALS
- Field-Flow Fractionation : MALS オ モチイタ スイヨウセイ コウブンシ ノ ブンシ トクセイ カイセキ
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抄録
Field-flow fractionation (FFF) is one of the promising alternative separation techniques to size exclusion chromatography (SEC). In this study, water soluble polymers including Bovine Serum Albumin (BSA), pullulan, poly(ethylene oxide) (PEO), sodium poly(styrene sulfonate) (NaPSS), and partially saponified poly(vinyl alcohol) (PVA) have successfully been characterized by a means of the FFF technique, fitted with a multiangle laser light scattering (MALS) detector in 0.1 M Na NO3 as an eluent at 25°C. The factors which affect the retention time, tr, and the separation factor, Rs, of the monomer and dimer of BSA are the thickness of spacer, w, channel flow rate, VCHF, cross-flow rate, VCRF, focusing time, injection volume, and polymer concentration, Cp. We found that the retention time, tr, in the FFF follows the equation of tr~[VCHF]−1.0[VCRF]1.0[w]2.0, in agreement with theory. Several cross-flow rates were examined for the characterization of the water soluble flexible polymers and the polyelectrolyte, and an appropriate gradient method for these polymers is found to be described by the equation of VCRF = A exp (−Btr), where A and B are constant. For the polyelectrolyte, the retention time significantly varies with salt concentration, due to the balance between change in the conformation of polyelectrolyte chains and the electrostatic exclusion from the membrane filter.
収録刊行物
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- 高分子論文集
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高分子論文集 70 (2), 72-81, 2013
公益社団法人 高分子学会
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詳細情報 詳細情報について
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- CRID
- 1390001206526700544
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- NII論文ID
- 10031155066
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- NII書誌ID
- AN00085011
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- ISSN
- 18815685
- 03862186
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- NDL書誌ID
- 024295013
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
