Autocrine action of transforming growth factor-.BETA.1 (TGF-.BETA.1) in the invasiveness of human oral squamous cell carcinoma cell lines.
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- OKUMURA Kazuhiko
- First Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- KONISHI Akira
- First Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- TANAKA Maki
- First Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- HAGINO Tsukasa
- First Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- NAGAYASU Hiroki
- Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- KATOH Motoyasu
- Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- KAWANO Hajime
- Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- SHIBATA Toshiyuki
- Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
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- KANAZAWA Masaaki
- First Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido
Bibliographic Information
- Other Title
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- 培養ヒト口腔へん平上皮癌細胞の浸潤におけるTransforming growth factor-β1(TGF‐β1)のオートクリン作用
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Abstract
We studied the possible role of transforming growth factor-β 1 (TGF-β 1) in tumor invasiveness by analyzing the production and activation of TGF-β 1 in eight human oral squamous cell carcinoma cell lines.<BR>The rate of secretion of TGF-β 1 was measured with a [125I]-TGF-β 1 radioreceptor assay. All human oral squamous cell carcinoma cells secreted TGF-β 1 into the culture media. Three cell lines secreted very high levels of activated TGF-19.1. When the invasion of a monolayer of lung endothelial cells was assayed in vitro, highly invasive cell lines were found to secrete activated TGF-β 1 at higher rates than weakly invasive cell lines. These findings suggested that the different invasiveness of these cell lines was associated with the rate of activated TGF-β 1 production. Highly invasive potential squamous cell carcinoma cells, such as SAS, Ca 9 -22, and OSC-20, were inhibited by treatment with neutralizing anti TGF-β 1-antibody. Furthermore, the invasiveness of weakly (T. T and HSC-2) and highly (SAS) invasive cells was enhanced by SAS culture media. Affinity labeling of [125I]-TGFβ 1 to cell surface receptors revealed the two major affinity crosslinked bands (type I and II I). These experiments indicate that TGF-β may modulate the invasive potential of human oral squamous cell carcinoma cells by autocrine action.
Journal
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- Japanese Journal of Oral and Maxillofacial Surgery
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Japanese Journal of Oral and Maxillofacial Surgery 42 (10), 962-968, 1996
Japanese Society of Oral and Maxillofacial Surgeons
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Details 詳細情報について
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- CRID
- 1390001206529523968
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- NII Article ID
- 10011659090
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- NII Book ID
- AN00189163
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- ISSN
- 21861579
- 00215163
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed