培養ヒト口腔へん平上皮癌細胞の浸潤におけるTransforming growth factor-β1(TGF‐β1)のオートクリン作用

  • 奥村 一彦
    北海道医療大学歯学部口腔外科学第一講座
  • 小西 亮
    北海道医療大学歯学部口腔外科学第一講座
  • 田中 真樹
    北海道医療大学歯学部口腔外科学第一講座
  • 萩野 司
    北海道医療大学歯学部口腔外科学第一講座
  • 永易 裕樹
    北海道医療大学歯学部口腔外科学第二講座
  • 加藤 元康
    北海道医療大学歯学部口腔外科学第二講座
  • 河野 峰
    北海道医療大学歯学部口腔外科学第二講座
  • 柴田 敏之
    北海道医療大学歯学部口腔外科学第二講座
  • 金澤 正昭
    北海道医療大学歯学部口腔外科学第一講座

書誌事項

タイトル別名
  • Autocrine action of transforming growth factor-.BETA.1 (TGF-.BETA.1) in the invasiveness of human oral squamous cell carcinoma cell lines.

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We studied the possible role of transforming growth factor-β 1 (TGF-β 1) in tumor invasiveness by analyzing the production and activation of TGF-β 1 in eight human oral squamous cell carcinoma cell lines.<BR>The rate of secretion of TGF-β 1 was measured with a [125I]-TGF-β 1 radioreceptor assay. All human oral squamous cell carcinoma cells secreted TGF-β 1 into the culture media. Three cell lines secreted very high levels of activated TGF-19.1. When the invasion of a monolayer of lung endothelial cells was assayed in vitro, highly invasive cell lines were found to secrete activated TGF-β 1 at higher rates than weakly invasive cell lines. These findings suggested that the different invasiveness of these cell lines was associated with the rate of activated TGF-β 1 production. Highly invasive potential squamous cell carcinoma cells, such as SAS, Ca 9 -22, and OSC-20, were inhibited by treatment with neutralizing anti TGF-β 1-antibody. Furthermore, the invasiveness of weakly (T. T and HSC-2) and highly (SAS) invasive cells was enhanced by SAS culture media. Affinity labeling of [125I]-TGFβ 1 to cell surface receptors revealed the two major affinity crosslinked bands (type I and II I). These experiments indicate that TGF-β may modulate the invasive potential of human oral squamous cell carcinoma cells by autocrine action.

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