Application of spectrophotometry to estimate the optimum culture conditions for <I>Nannochloropsis oculata</I> as a diet for zooplankton

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  • <I>Nannochloropsis oculata</I> のバッチ培養における動物プランクトンの餌料として好適な培養状態を推定するための分光光度法の応用
  • Application of spectrophotometry to estimate the optimum culture conditions for Nannochloropsis oculata as a diet for zooplankton

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Abstract

Counting cells is a difficult way to estimate the optimum culture conditions for Nannochloropsis oculata as a feed for rotifers efficiently. To devise a simple tool that could be used quickly to estimate the optimum harvest time using spectrophotometry, we investigated the relationship between N. oculata growth population patterns, absorption spectra and nutrient salt concentrations. The cell densities, absorption values at each wavelength (360-780 nm) and concentrations of nitrate-nitrogen and phosphate were measured daily. The logarithmic phase was terminated just before phosphate limitation. The absorption ratio of 490 nm per 680 nm increased until the start of the logarithmic phase, continued to decrease beyond the logarithmic phase and then increased again just after phosphate starvation. The cells reached a stationary phase after additional nitrate-nitrogen starvation. These results suggest that absorption spectra change the trend gradually in response to nutrient salt starvation rather than to growth phase transition. The present study suggests, the possibility of applying spectrophotometry to monitor N. oculata culture conditions more accurately than the cell count method allows.

Journal

  • Aquaculture Science

    Aquaculture Science 65 (3), 209-219, 2017-09-20

    Japanese Society for Aquaculture Science

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