Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus <i>Gekko</i> (Squamata: Gekkonidae)

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  • OKAMOTO Kota
    Graduate School of Engineering and Science, University of the Ryukyus
  • KURITA Takaki
    Chiba Biodiversity Center
  • NAGANO Masahiro
    Faculty of Science and Technology, Oita University
  • SATO Yukuto
    Center for Strategic Research Project, Organization for Research Promotion, University of the Ryukyus
  • AOYAMA Hiroaki
    Research Planning Office, Organization for Research Promotion, University of the Ryukyus
  • SAITOH Seikoh
    College of Economics and Environmental Policy, Okinawa International University
  • SHINZATO Naoya
    Tropical Biosphere Research Center, University of the Ryukyus
  • TODA Mamoru
    Tropical Biosphere Research Center, University of the Ryukyus

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  • Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus Gekko (Squamata: Gekkonidae)

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<p>Gekko yakuensis and G. tawaensis, both endemic to western Japan, are threatened by genetic introgression from G. hokouensis and G. japonicus, respectively. To know detailed situation of their hybridizations for planning relevant conservation measures, development of sensitive genetic markers is desired. We here developed microsatellite markers based on the sequences obtained from G. hokouensis using 454 GS Junior sequencer, and tested stability of PCR amplification and species-specificity of alleles at each locus using G. hokouensis, G. yakuensis, G. japonicus, and G. tawaensis. The results showed that 22 loci were almost constantly amplified in more than one species. We further confirmed that there were fixed or nearly fixed allelic displacement between G. hokouensis and G. yakuensis, and between G. japonicus and G. tawaensis at 14 loci. Thus, these 22 loci are considered to be useful for evaluation of hybridizations between these pairs of species.</p>

Journal

  • Current Herpetology

    Current Herpetology 39 (1), 66-74, 2020

    The Herpetological Society of Japan

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