Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus Gekko (Squamata: Gekkonidae)
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- OKAMOTO Kota
- Graduate School of Engineering and Science, University of the Ryukyus
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- KURITA Takaki
- Chiba Biodiversity Center
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- NAGANO Masahiro
- Faculty of Science and Technology, Oita University
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- SATO Yukuto
- Center for Strategic Research Project, Organization for Research Promotion, University of the Ryukyus
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- AOYAMA Hiroaki
- Research Planning Office, Organization for Research Promotion, University of the Ryukyus
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- SAITOH Seikoh
- College of Economics and Environmental Policy, Okinawa International University
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- SHINZATO Naoya
- Tropical Biosphere Research Center, University of the Ryukyus
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- TODA Mamoru
- Tropical Biosphere Research Center, University of the Ryukyus
書誌事項
- タイトル別名
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- Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus <i>Gekko</i> (Squamata: Gekkonidae)
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説明
<p>Gekko yakuensis and G. tawaensis, both endemic to western Japan, are threatened by genetic introgression from G. hokouensis and G. japonicus, respectively. To know detailed situation of their hybridizations for planning relevant conservation measures, development of sensitive genetic markers is desired. We here developed microsatellite markers based on the sequences obtained from G. hokouensis using 454 GS Junior sequencer, and tested stability of PCR amplification and species-specificity of alleles at each locus using G. hokouensis, G. yakuensis, G. japonicus, and G. tawaensis. The results showed that 22 loci were almost constantly amplified in more than one species. We further confirmed that there were fixed or nearly fixed allelic displacement between G. hokouensis and G. yakuensis, and between G. japonicus and G. tawaensis at 14 loci. Thus, these 22 loci are considered to be useful for evaluation of hybridizations between these pairs of species.</p>
収録刊行物
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- Current herpetology
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Current herpetology 39 (1), 66-74, 2020
日本爬虫両棲類学会
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詳細情報 詳細情報について
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- CRID
- 1390002184877653120
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- NII論文ID
- 130007802503
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- NII書誌ID
- AA12246863
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- ISSN
- 18811019
- 13455834
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- NDL書誌ID
- 030277098
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可