Verification of Sample Collection Sites for PCR-based Detection Assay for Abalone Asfa-like Virus

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  • Matsuyama Tomomasa
    Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
  • Kiryu Ikunari
    Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
  • Inada Mari
    Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
  • Nakayasu Chihaya
    Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency

Bibliographic Information

Other Title
  • PCRによるAbalone asfa-like virusの検出に適した検体採取部位の検証
  • PCR ニ ヨル Abalone asfa-like virus ノ ケンシュツ ニ テキシタ ケンタイ サイシュ ブイ ノ ケンショウ

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Abstract

<p>We measured gene quantities of the abalone asfa-like virus (AbALV), which is presumed to be the pathogen of abalone amyotrophia, in various tissues of affected Japanese abalone (Haliotis discus discus) with a quantitative PCR. ​Large quantities of the gene were detected in any tissues or swabs from the body surface. ​No significant difference in the gene quantity was found among tissues, although the gene quantity in the hemolymph was lower. ​The virus was also detected by a conventional PCR from affected animals. ​These results indicate that both PCRs are capable of the detection of AbALV, in any tissues by the destructive test and in body surface mucus by the non-destructive test.</p>

Journal

  • Fish Pathology

    Fish Pathology 56 (1), 18-21, 2021-03-15

    The Japanese Society of Fish Pathology

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