Verification of Sample Collection Sites for PCR-based Detection Assay for Abalone Asfa-like Virus
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- Matsuyama Tomomasa
- Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
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- Kiryu Ikunari
- Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
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- Inada Mari
- Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
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- Nakayasu Chihaya
- Pathology Division, Aquaculture Research Department, National Research and Development Agency, Japan Fisheries Research and Education Agency
Bibliographic Information
- Other Title
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- PCRによるAbalone asfa-like virusの検出に適した検体採取部位の検証
- PCR ニ ヨル Abalone asfa-like virus ノ ケンシュツ ニ テキシタ ケンタイ サイシュ ブイ ノ ケンショウ
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Abstract
<p>We measured gene quantities of the abalone asfa-like virus (AbALV), which is presumed to be the pathogen of abalone amyotrophia, in various tissues of affected Japanese abalone (Haliotis discus discus) with a quantitative PCR. Large quantities of the gene were detected in any tissues or swabs from the body surface. No significant difference in the gene quantity was found among tissues, although the gene quantity in the hemolymph was lower. The virus was also detected by a conventional PCR from affected animals. These results indicate that both PCRs are capable of the detection of AbALV, in any tissues by the destructive test and in body surface mucus by the non-destructive test.</p>
Journal
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- Fish Pathology
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Fish Pathology 56 (1), 18-21, 2021-03-15
The Japanese Society of Fish Pathology
