Development of Automatic Immunosensor System for Quantification of Allergens

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  • Higuchi Motonobu
    Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
  • Kuwano Takahisa
    Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
  • Matsumoto Kiyoshi
    Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University

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Other Title
  • アレルゲン自動計測用免疫センサーシステムの開発
  • アレルゲン ジドウ ケイソクヨウ メンエキ センサー システム ノ カイハツ

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Abstract

A glassy carbon electrode, anodized for a short time at about 2.0 V vs. Pt，is a very convenient regenerable solid phase for the successive steps of an automated enzymelinked immunosorbent assay. The anodized surface absorbs allergen and the blocking proteins in a similar manner to classical polystyrene solid phase; the detection of the immobilized alkaline phosphatase used as a label can be achieved from an enzymatic process occurring; and the protein stacking, accumlated during the assay, can be eliminated by a simple re-anodization at the end of the cycle, allowing the adsorption of a new allergen. Using peroxidase or alkaline phosphatase as labels, various allergens such as ovalbumin, ovomucoid, and β-lactoglobulin were assayed.

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