油中水滴エマルションで調製するジャイアントベシクルに対する油性分散媒の影響

  • 豊田 太郎
    東京大学大学院総合文化研究科広域科学専攻 東京大学生物普遍性連携研究機構
  • 章 逸汀
    東京大学大学院総合文化研究科広域科学専攻

書誌事項

タイトル別名
  • Effect of an Oil Medium on Giant Vesicles Prepared with Water-in-Oil Emulsion

抄録

<p>A closed lipid bilayer membrane with a diameter of one micrometer or more in water is called as giant vesicle and it is attracting attention as a cell mimicry reaction space because its structure and size are similar to those of living cells. When utilizing giant vesicles from a cell model perspective, biomolecules are needed to be encapsulated inside giant vesicles under conditions close to the cytoplasm. In recent years, the development of this encapsulation technology has been accelerating. The origin of this trend is a preparation method of giant unilamellar vesicles using a water-in-oil emulsion, published by a research group at Harvard University in 2003. The preparation method can only be performed on a desktop centrifuge. Here, we report on the experimental results of this preparation method using different oil for the water-in-oil emulsion.</p>

収録刊行物

  • 分析化学

    分析化学 71 (1.2), 83-89, 2022-01-05

    公益社団法人 日本分析化学会

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