書誌事項
- タイトル別名
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- Evaluation of the impact of carryover in a fully automated, high-sensitivity hepatitis B core-related antigen assay
- コウカンド HB コア カンレン コウゲン ゼン ジドウ ソクテイケイ ニ オケル キャリーオーバー ノ ケンショウ
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抄録
<p>We developed and reported a fully automated, high-sensitivity hepatitis B core-related antigen assay (iTACT-HBcrAg). This study aimed to evaluate the impact of carryover during the use of iTACT-HBcrAg by analyzing samples with high HBcrAg levels. A negative sample tested immediately after a sample with an HBcrAg of 8.7 LogU/mL showed an HBcrAg of 2.4 LogU/mL, thus confirming carryover. The sample with an HBcrAg of 8.8 LogU/mL and collection tubes containing the negative samples were continuously opened to check for the effects of sample splashing and aerosols. The results showed that sample splashing and aerosols did not account for the carryover. The iTACT-HBcrAg is a highly sensitive assay; therefore, carryover can occur when magnetic particle-antigen complexes are introduced into the next sample via the BF wash nozzle. Any sample determined to be positive for HBcrAg after the testing of a sample with an HBcrAg above the measurement range should be retested for confirmation.</p>
収録刊行物
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- 肝臓
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肝臓 64 (12), 656-659, 2023-12-01
一般社団法人 日本肝臓学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390016958307513728
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- NII書誌ID
- AN00047770
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- ISSN
- 18813593
- 04514203
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- NDL書誌ID
- 033212516
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可