フローインジェクション分析法による生体試料中のタンパク質の迅速高感度定量

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  • Rapid and sensitive determination of proteins in biological samples by FIA.
  • フロー インジェクション ブンセキホウ ニ ヨル セイタイ シリョウチュウ ノ

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A method for the determination of proteins in biological samples was developed by combining FIA with a Coomassie Brilliant Blue G-250(CBB G-250) dyebinding method. This system consists of two delivery pumps of reagent solution and carrier solution, a sample injector, a reaction coil and a spectrophotometer. The wavelength of the spectrophotometer was set at 600 nm and 0.1 m of reaction coil(1 mm i.d.) was used. Each flow rate of the reagent solution and carrier solution was 1.0 ml/min. The concentration of 60 μg/ml of CBB G-250 and 2.7% of perchloric acid in the reagent solution gave the maximum sensitivity. In the carrier solution 20 μl of the sample was injected. A linear calibration curve was obtained from 40 ng up to 1.0 μg of bovine serum albumin (BSA) as a standard substance. The minimum detectable amount of protein was 40 ng and the relative standard deviation for 1.0 μg of BSA (n=10) was 0.2%. This method allowed the analysis of 60 samples/h. Proteins in rat liver microsome were determined by this method. The analytical results showed good correlations with those by the manual method using CBB G-250 and by Lowry's manual method.

収録刊行物

  • 分析化学

    分析化学 36 (4), 256-260, 1987

    公益社団法人 日本分析化学会

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