Micro-fabrication and analytical performances of quartz and glass microchips for electrophoresis.

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  • マイクロマシニング技術を用いる石英及びガラス製電気泳動チップの作製とそれらの基本特性評価
  • マイクロマシニング ギジュツ オ モチイル セキエイ オヨビ ガラスセイ デン

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Abstract

Microchips for capillary electrophoresis have been fabricated in small size on quartz or glass substrates. Each microchip is 11 mm × 28 mm in size and 1.5 mm in thickness. It has a sample loading channel, a separation channel and four reservoirs at the ends of each channel. To fabricate a quartz chip, we applied a novel bonding method with a 1% (w/v) hydrofluoric acid solution. Using fabricated microchips, we evaluated several of their analytical features. We have shown that the microchips permit quantitative sample injection (several 10 pl or less) with an adequate potential control, high-speed analysis (30 s) due to the high heat-dissipation capability and effective separation (the number of theoretical plates is over 2500 at separation length of 17 mm).

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 47 (6), 361-368, 1998

    The Japan Society for Analytical Chemistry

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