Separation and determination of a peroxide mixture by HPLC with postcolumn detection.

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  • YAMADA Kaoru
    Lion Corporation,7-13-12,Hirai,Edogawa-ku,Tokyo 132-0035
  • MATSUTANI Shigeaki
    Lion Corporation,7-13-12,Hirai,Edogawa-ku,Tokyo 132-0035
  • HORIGUCHI Yasunobu
    Lion Corporation,7-13-12,Hirai,Edogawa-ku,Tokyo 132-0035
  • HOBO Toshiyuki
    Department of Applied Chemistry,Graduate School of Engineering,Tokyo Metropolitan University,1-1,Minami-Ohsawa,Hachioji-shi,Tokyo 192-0397

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Other Title
  • ポストカラム検出高速液体クロマトグラフィーによる過酸化物の分別定量
  • ポストカラム ケンシュツ コウソク エキタイ クロマトグラフィー ニ ヨル カサンカブツ ノ フンベツ テイリョウ

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Description

A simple and rapid method using HPLC with postcolumn detection has been developed for the separation and determination of a peroxide mixture.A hydrogen peroxide(H2O2), methylhydroperoxide(MHP)and peroxosulfate mixture was chosen for the sample.LC separation was carried out on an Inertsil ODS-2 column(4.6mm i.d.×250mm length)using pH-adjusted distilled water with H3PO4 as the mobile phase at a flow rate of 1ml/min.An oxidizing reaction of I- by peroxide was applied to the postcolumn reaction.Thus, a 10% KI aq.solution at a flow rate of 0.5ml/min was used as the reaction solution.Teflon tubing(0.5mm i.d.×10m length)was used as a reaction coil at 90°C.UV detection was performed at 348nm.The detection limits(S/N=3)for H2O2 and MHP were 35ppb and 50ppb, respectively.Under this analytical condition, peroxides and I- reacted quantitatively.Thus, the determination of various kinds of peroxides is possible by comparing the peak areas with that of a H2O2 standard solution, even if the standard substance of the peroxides is not available.Since the proposed system uses only easily available apparatus and reagents, this method is suitable for process control.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 49 (8), 587-591, 2000

    The Japan Society for Analytical Chemistry

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