書誌事項
- タイトル別名
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- Growth of Protein Crystals in Hydrogels with High Strength
- ジッケンシツ コソウ ハイドロゲル チュウ デ ノ タンパクシツ ケッショウカ
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High-throughput X-ray protein crystallography offers unprecedented facilitation to structure based drug discovery. The popular way to prepare crystals of target protein in complex with a candidate compound is the soaking method. However, the method often causes damage due to osmotic pressure to crystals, especially when a compound is dissolved in an organic solvent or solution with high ionic strength. These solutions damage protein crystals by osmotic shock. To overcome this difficulty, we have developed a novel technique to grow protein crystals in the concentrated hydrogel. Here, we demonstrate that protein crystals grown in hydrogel are stable to soaking and suffer less damage.
収録刊行物
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- 日本結晶学会誌
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日本結晶学会誌 54 (5), 300-303, 2012
日本結晶学会
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詳細情報 詳細情報について
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- CRID
- 1390282679065341824
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- NII論文ID
- 10031129966
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- NII書誌ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL書誌ID
- 024085233
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可