Rapid Separation of Gangliosides Using Strong Anion Exchanger Cartridges
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- Kato Tomohisa
- Japan Chemical Innovation Institute Kaneka Corporation
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- Kasuya Maria Carmelita Z.
- Institute of Industrial Science, The University of Tokyo
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- Hatanaka Kenichi
- Institute of Industrial Science, The University of Tokyo
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説明
A method utilizing strong anion exchanger cartridges (InertSep SAX) was developed to separate gangliosides. Total lipids extracted from rat brain is able to be rapidly separated into neutral and acidic lipids rapidly. Neutral lipids were passed through the SAX cartridge while acidic lipids adsorbed onto the cartridge and were eluted by chloroform/methanol/4.0 M aqueous ammonium acetate (5:10:1, by volume). Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) were separated individually according to their characteristics by elution with increasing concentration of ammnonium acetate (0 - 4.0 M). The gangliosides yield of this procedure was higher than 95%.<br>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 57 (7), 397-400, 2008
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679068784256
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- NII論文ID
- 130000055388
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
- http://id.crossref.org/issn/13458957
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- NDL書誌ID
- 9536391
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可