Alkinonase A and AF, New Alkaline Proteinases produced by <I>Streptomyces violaceorectu8</I>

NAKAMURA SHOSHIRO, FUKUDA HIDEO, YAMAMOTO TAKUZO, OGURA MICHIKO, HAMADA MASA, MATSUZAKI MEIKI, UMEZAWA HAMAO

doi:10.1248/cpb.20.385

Alkinonase A and AF, New Alkaline Proteinases produced by <I>Streptomyces violaceorectu8</I>

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NAKAMURA SHOSHIRO

Pharmaceutical Institute, Hiroshima University School of Medicine
FUKUDA HIDEO

Pharmaceutical Institute, Hiroshima University School of Medicine
YAMAMOTO TAKUZO

Institute of Microbial Chemistry
OGURA MICHIKO

Institute of Microbial Chemistry
HAMADA MASA

Institute of Microbial Chemistry
MATSUZAKI MEIKI

Institute of Microbial Chemistry
UMEZAWA HAMAO

Institute of Microbial Chemistry

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説明

Alkaline proteinases, named alkinonase A and AF, are isolated from the cultured broth of Streptomyces violaceorectus. Both alkinonases show remarkably strong antiinflammatory activity for the carrageenin-induced edema. Alkinonase A and AF are belonging to a new type of alkaline proteinases of microbial origin. The effect of inhibitors on the proteolytic activity and the substrate specifities of alkinonases are resembled to that of the neutral proteinases of microbial origin and different from that of the alkaline proteinases of microbial origin. Production, isolation, purification, effect of pH on the proteolytic activity, pH stability, effect of temperature on the proteolytic activity, effect of inhibitors on the proteolytic activity, substrate specifities and anti-inflammatory activity for the carrageenin induced edema of alkinonases are described in this paper.