Agarose-2D-DIGE: Fluorescent 2-D differential gel electrophoresis (2-D DIGE) with agarose gels in the first-dimensional isoelectric focusing (IEF)

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  • 一次元目にアガロースゲル等電点電気泳動を用いた蛍光ディファレンスゲル二次元電気泳動
  • 1ジゲンメ ニ アガロース ゲル トウデンテン デンキ エイドウ オ モチイタ ケイコウ ディファレンス ゲル 2ジゲン デンキ エイドウ

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A two-dimensional gel electrophoresis (2-DE) method that uses an agarose isoelectric focusing (IEF) gel in the first dimension (agarose 2-DE) offers advantages over the more widely used immobilized pH gradient 2-DE for separating high-molecular-mass proteins (100-500kDa) and for having a higher loading capacity (1.5mg in total). We have applied the Fluorescent 2-D differential gel electrophoresis (2-D DIGE) to our agarose 2-DE system. This allowed us to see clear differences in the 2-DE patterns from liver extracts of a diabetic model Otsuka Long-Evans Tokushima Fatty (OLETF) and its control Long-Evans Tokushima Otsuka (LETO) rats including changes in the amounts of several proteins larger than 100kDa. The combined method would increase its power to detect changes in disease proteomics.

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