Purification and properties of .ALPHA.1- and .ALPHA.2-macroglobulins in the dog.

DOI

抄録

1) The ultrafiltration of serum collected from dogs by a filtration film with a pore size of 300, 000, Blue Sepharose CL-6B affinity chromatography and Ultrogel AcA 22gel filtration chromatography eluted a third protein fraction in addition to α1-macroglobulin (α1-M) and α2-macrogrobulin (α2-M). The subsequent use of preparative agarose gel isoelectric focusing with a cover gel facilitated the extraction of pure α1-M and α2-M protein fractions.2) Analytical agarose gel isoelectric focusing and Ferguson plot by agarose gel electrophoresis revealed the following: (1) dog α1-M had an isoelectric point (pI) of 5.4 and a molecular weight of 724, 000; and (2) dog α2-M had a pI of 5.7 and a molecular weight of 758, 000.

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キーワード

詳細情報 詳細情報について

  • CRID
    1390282679178644736
  • NII論文ID
    130003606935
  • DOI
    10.2198/sbk.36.139
  • ISSN
    13499785
    00319082
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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