アガロースゲル電気泳動におけるＬＤＨアイソザイムの発色条件の改良

DOI
  • 黒田 五夫
    国家公務員等共済組合連合会立川病院・中央検査部・生化学
  • 中沢 千浪
    国家公務員等共済組合連合会立川病院・中央検査部・生化学
  • 森 皎祐
    国家公務員等共済組合連合会立川病院・内科
  • 吉田 光孝
    東邦大学・理学部生理化学

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  • Color developmental conditions for LDH isoenzyme assay by agarose gel electrophoresis.

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Color developmental condition in LDH isoenzyme assay using agarose gel film as medium was investigated fundamentally. Optimum conditions for this assay were pH8.6, DL-litium lactate concentration 120∼150mM/l, NAD concentration 4∼5mM/l and tricine concentration 0.1M/l. These conditions were found to be identical for those of Pol-E film method. However, optimum concentrations of diaphorase enzyme used as an intermediate electron carrier was 7, 500U/l and that of NTB was 0.5mM/l. Furthermore, the color developmental reaction in agarose gel film was a linear at least in 60 minutes. After these improvements, an assay condition with better color developmental sensibility and quantitability was obtained in comparison with Pol-E film method.

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