DNA methylation in normal colon—especially about "field cancerization"
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- Maeda Osamu
- Advanced Research of Gastroenterology, Nagoya University Graduate School of Medicine
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- Ando Takafumi
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- Ishiguro Kazuhiro
- Advanced Research of Gastroenterology, Nagoya University Graduate School of Medicine
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- Watanabe Osamu
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- Hibi Yoko
- Department of Hospital Pharmacy, Nagoya University Graduate School of Medicine
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- Nagai Taku
- Department of Hospital Pharmacy, Nagoya University Graduate School of Medicine
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- Yamada Kiyofumi
- Department of Hospital Pharmacy, Nagoya University Graduate School of Medicine
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- Funasaka Kohei
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- Miyahara Ryoji
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- Ohymiya Naoki
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- Goto Hidemi
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
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- J. Issa Jean-Pierre
- Fels Institute for Cancer and Molecular Biology, Temple University
Bibliographic Information
- Other Title
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- DNAメチル化と正常大腸―特にfield cancerizationについて―
- DNA methylation in normal colon^|^mdash;especially about ^|^quot;field cancerization^|^quot;
Description
Phenomenon of field cancerization or field defect is biological change as premalignancy at non-cancerous region around tumor, which is considered to be an important mechanism of carcinogenesis. We evaluated whether normal appearing mucosa of colorectal cancer patients has aberrant DNA methylation in a genome-wide manner. We compared methylation status in normal colorectal mucosa of healthy persons and colorectal cancer patients using methylated CpG island amplification microarray (MCAM), and extracted candidate genes with difference in methylation levels between healthy persons and colorectal cancer patients. We extracted fifteen genes in which we judged to have difference of methylation using MCAM between four pairs of normal colorectal mucosa of healthy persons and normal appearing mucosa adjacent to colorectal cancer. We performed quantitative methylation analysis with bisulfite pyrosequencing for candidate genes extracted with MCAM for the four pairs of samples. We also estimated the methylation levels of the genes of 50 colorectal biopsy samples using quantitative methods. We did not find any genes that revealed significant difference in methylation levels between adjacent mucosa and healthy normal colon. Aberrant promoter hypermethylation in normal appearing mucosa adjacent to colorectal cancer is not considered to be common.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 56 (1), 9-14, 2012
Japanese Electrophoresis Society
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Details 詳細情報について
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- CRID
- 1390282679179636480
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- NII Article ID
- 130004456515
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- DOI
- 10.2198/sbk.56.9
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- COI
- 1:CAS:528:DC%2BC3sXjtVSnsr8%3D
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- ISSN
- 13499785
- 00319082
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed