Cytological Studies on Micrococcus glutamicus

ITAGAKI Shiro, KOBATA Mamoru, KINOSHITA Shukuo

doi:10.15281/jplantres1887.74.498

It was reported in a previous paper that the elongated, enlarged and even branched form of Micrococcus glutamicus was observed in the synthetic medium containing Na-citrate or Na-malate. As the result of the investigations on substances having the effect like that of Na-citrate or Na-malate, following informations were obtained obviously. 1. Among various salts of citrate, ammonium and potassium salts showed the similar effect to that of Na-salt on the morphological change. 2. Na-oxalate also has a strong elongating and branching effect. 3. Generally, amino acids promote the cell growth of M. glutamicus. The large quantities of L-alanine and L-cystein, however, inhibit its growth. The elongated and enlarged cells were formed by the addition of 50mg./ml. of L-cystein. 4. High multicellular and elongated bodies were formed in a medium containing the low level of penicillin. Such multicellular bodies, however, were never formed in the penicillinmedia if the biotin content was increased to 100γ/l. From this fact, it seems that penicillin strongly inhibits the separation of the daughter cells rather than the cytoplasmic division. 5. The multicellular bodies never formed in a medium containing streptomycin. But a vacuole-like structure in each cell was formed in the concentration of 0.4-0.8γ/ml. of streptomycin. 6. Remarkable multicellular bodies were formed in the concentration of 0.2-0.8γ/ml. of mitomycin-C. In these cases, the length of cell is 10μ or over occassionally. One or both ends of these cells were swollen and become so-called club-shape.

Cytological Studies on <i>Micrococcus glutamicus</i>

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