書誌事項
- タイトル別名
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- Methods for determination of Milt Protein and .EPSILON.-Polylysine in Food Additive Preparations and Processed Foods by Capillary Zone Electrophoresis.
- Methods for Determination of Milt Protein and ε-Polylysine in Food Additive Preparations and Processed Foods by Capillary Zone Electrophoresis
- Methods for Determination of Milt Protein and イプシロン Polylysine in Food Additive Preparations and Processed Foods by Capillary Zone Electrophoresis
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抄録
A simple and rapid method using capillary zone electrophoresis (CZE) for the determination of milt protein (MP), which contains mainly protamine, and polylysine (PL) in food additive preparations and processed foods was developed. CZE separation was performed on poly(vinyl alcohol)-coated capillaries at a column temperature of 20°C with 120 mmol/L phosphate buffer (pH 2.5) as the running buffer.<br>The influence of various components in food additive preparations on CZE analysis of MP and PL was examined. Egg white lysozyme, glycine, sodium acetate, glycerol, fumaric acid, calcium carbonate, dextrin, emulsifiers and sodium polyphosphate and pyrophosphate had no effect. No peak of protamine was detected in preparations containing metaphosphate.<br>The analysis method for processed foods was composed of extraction with 4% formic acid, precipitation of macromolecular compounds with ethanol, concentration in a water bath and determination by CZE. The average recoveries were 108.4% for protamine sulfate (PS) in red bean sticky rice, and 81.3% for PL in white rice, 118% in egg sandwiches, and 115% in shiraae. The limits of detection of PS in red bean sticky rice and PL in white rice were both 50 ppm.
収録刊行物
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- 食品衛生学雑誌
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食品衛生学雑誌 42 (2), 79-83, 2001
公益社団法人 日本食品衛生学会
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詳細情報 詳細情報について
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- CRID
- 1390282679199856768
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- NII論文ID
- 10009305860
- 130000671012
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- NII書誌ID
- AN00117741
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- COI
- 1:CAS:528:DC%2BD3MXjs1CltL8%3D
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- ISSN
- 18821006
- 00156426
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- NDL書誌ID
- 5754798
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- PubMed
- 11486387
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可