組織切片によるャヌスグリーンB還元とミトコンドリャ染色の機構について
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- 佐藤 七郎
- 東京大学理学部植物学教室細胞学研究室
書誌事項
- タイトル別名
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- On the Reduction of Janus Green B by Plant Embryo Slices and the Mechanism of the Specific Stainiug of Mitochondria
抄録
Janus green B stains the periderm and the pith of hypocotyl and radicle of ungerminated seeds of Phaseolus vulgaris. The procambium, which contains no mitochondria, is not stained from beginning to end of the procedure unless the cells are living Under a coverglass, the stain reduced and decolorized. This reduction is inhibited by heating (52°C for 30min.), low pH, acetate buffer (M/10), malonate monoiodoacetate (M/1000), arsenite (M/300), and Pb (NO3)2 (M/10), and accelerated (M/100), by succinate (6M/10). These inhibitions and acceleration show that succinic dehydrogenase plays a role in the process of the reduction of Janus green B, presumably in directly under the present experimental conditions. Cyanide (M/1000) also inhibits the reduction. Without coverglass, i. e. in complete aerobic condition, the reduction dose not occur whether cyanide is present or not.<br>The hypothesis of L Aazarow and Cooperstein on the mechanism of the specific staninig of mitochondria was criticized on the ground of the above stated experimental data and some theoretical considerations.
収録刊行物
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- 植物学雑誌
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植物学雑誌 69 (812), 87-90, 1956
公益社団法人 日本植物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679201126400
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- NII論文ID
- 130004212294
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- ISSN
- 21853835
- 0006808X
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可