Evaluation of Selective Plating Media for the Detection of Heat- or Freeze-injured Staphylococcus aureus.

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  • 加熱及び凍結損傷黄色ブドウ球菌検出のための選択分離培地の検討
  • カネツ オヨビ トウケツ ソンショウ キイロ ブドウ キュウキン ケンシュツ ノ タメ ノ センタク ブンリバイチ ノ ケントウ

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The efficacy of Baird-Parker (BP) agar, mannitol-salt-egg yolk (MSEY) agar and mannitol salt (MS) agar in detecting Staphylococcus aureus FRI-100 heated at 52°C for 20 min in 100 mmol/L potassium phosphate buffer was determined. Brain heart infusion agar with 1% pyruvate (BHIP agar) supported the highest recovery of injured cells and was used as the control medium.<br>Of the three selective media, significantly higher recovery of heat-injured cells was observed on BP agar than MSEY agar, and the poorest recovery was observed on MS agar (p<0.05). Low recovery of unheated cells was obtained for MS compared with other media (p<0.05). A reduction in populations occurred gradually in reagent-grade water stored for 14 days at -20°C. There was no significant difference between BHIP agar and MS agar in the number of freeze-injured cells recovered from 1 to 14 days.

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