Detection of Norovirus RNA in Bivalve Molluscs by Using Bacteria-Culture-Employed Method (A3T Method)

AKIBA Tetsuya, TANAKA Tatsuya, NAGANO Miyuki, MORI Kohji, HAYASHI Yukinao, OBATA Hiromi, CHIBA Takashi, IKUTA Yasuhisa, KAMIYA Yoriko, NAKAMA Akiko, HOSAKA Mitsugu, KAI Akemi

doi:10.3358/shokueishi.51.237

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細菌培養処理法（Ａ３Ｔ法）による二枚貝からのノロウイルス遺伝子の検出
サイキンバイヨウショリホウ A3Tホウニヨル 2マイガイカラノノロウイルスイデンシノケンシュツ

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Abstract

Norovirus (NV) RNA has rarely been detected in foods despite the use of highly sensitive methods such as RT-PCR and real-time RT-PCR. In the modified method (A3T method) reported previously, a bacterial culture process was introduced into the standard protocol for NV detection to remove some inhibitor(s) present in food ingredients. To confirm the efficiency of the A3T method and to examine NV contamination in bivalve molluscs, we tried to detect NV RNA in bivalve molluscs on the market and in oyster samples associated with foodborne outbreaks by using the standard method and the A3T method. NV RNAs were detected in 20 samples (18.0%) of 111 bivalve molluscs, including oysters, on the market by use of the A3T method, while only one sample (0.9%) was positive according to the standard method. NV RNA was also detected in 10 of 35 oyster samples related to foodborne outbreaks by the A3T method. Those results show that the A3T method is suitable for the detection of NV in bivalve molluscs in general laboratories.

Journal

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 51 (5), 237-241, 2010

Japanese Society for Food Hygiene and Safety

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