Measurement of Transport Activities of Bile Acids in Human Multidrug Resistance-Associated Protein 3 Using Liquid Chromatography-Tandem Mass Spectrometry
-
- YAMAGUCHI Kana
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
-
- MURAI Tsuyoshi
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
-
- YABUUCHI Hikaru
- Office of Collaborative Research and Technology Development, Kobe University
-
- KUROSAWA Takao
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
この論文をさがす
説明
A method has been developed for the measurement of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 3-expressing Sf9 cells for 1β-hydroxy-, 6α-hydroxy- and unsaturated bile acids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10 to 2000 pmol/mL, and the detection limit was less than 2 pmol/mL for all bile acids using selected reaction monitoring analysis. The method was applied to measurements of adenosine triphosphate-dependent transport activities of the membrane vesicles for the above-mentioned hydroxylated and unsaturated bile acids. The present study demonstrated that the human multidrug resistance-associated protein 3 vesicles accepted 1β-, 6α-hydroxylated and unsaturated bile acids along with common bile acids, such as glycocholic acid and taurolithocholic acid 3-sulfate. The developed method is useful for measurements of bile acid transport activities.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 26 (3), 317-323, 2010
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679232784384
-
- NII論文ID
- 10026782326
-
- NII書誌ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 10585586
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可