A Peptide Microarray for the Detection of Protein Kinase Activity in Cell Lysate
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- SHIGAKI Syuhei
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University
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- YAMAJI Takayuki
- Graduate School of Systems Life Sciences, Kyushu University
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- HAN Xiaoming
- Graduate School of Systems Life Sciences, Kyushu University
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- YAMANOUCHI Go
- Graduate School of Systems Life Sciences, Kyushu University
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- SONODA Tatsuhiko
- Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology
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- OKITSU Osamu
- Applied Genomics, Molecular Medicine Research Laboratories, Astellas Pharmaceuticals Inc.
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- MORI Takeshi
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
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- NIIDOME Takuro
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
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- KATAYAMA Yoshiki
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
書誌事項
- タイトル別名
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- Peptide Microarray for the Detection of Protein Kinase Activity in Cell Lysate
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説明
DNA microarray enables the analysis of DNA or mRNA expression levels, but it has not been possible to completely understand life using obtained information. Consequently, protein or peptide arrays have attracted much interest. Since the development of a practical protein microarray is still far away in light of handling difficulties, the peptide microarray is a promising tool for analyzing protein functions. We have developed a peptide microarray to detect protein kinase activity in cell lysate. All substrate peptides for kinases were immobilized chemoselectively on amino-coated glass slides. After phosphorylation of the immobilized peptides, phosphorylation was detected by fluorescence imaging. We detected the protein kinase activities, including that in cell lysate, in response to drug stimulation. Therefore, this peptide microarray would be useful for a high-throughput kinase assay of intracellular signals and would be applicable to drug screening.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 23 (3), 271-275, 2007
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233184896
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- NII論文ID
- 130004441418
- 10018823809
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2sXjtlektLk%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 8671729
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- PubMed
- 17372367
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- 本文言語コード
- en
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- NDLサーチ
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