A sensitive chemiluminescence method for the determination of H2O2 in exhaled breath condensate
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- HU Yufei
- Department of Chemistry, Institute of Analytical Science, Southwest University
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- ZHANG Zhujun
- Department of Chemistry, Institute of Analytical Science, Southwest University
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- YANG Chunyan
- Department of Chemistry, Institute of Analytical Science, Southwest University
書誌事項
- タイトル別名
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- A Sensitive Chemiluminescence Method for the Determination of H<sub>2</sub>O<sub>2</sub> in Exhaled Breath Condensate
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In this paper, a novel flow injection chemiluminescence (FI-CL) method is proposed for the determination of picomolar L-1 levels of hydrogen peroxide (H2O2) in exhaled breath condensate (EBC). This method is based on the oxidation of a low concentration of luminol (10-7 M) by H2O2 at a low concentration level (< 10-8 M) in an alkaline medium catalyzed by a complex, K5[Cu(HIO6)2] (DPC), which is not interfered by other metal ions or horseradish peroxidase (HRP). Under the optimum conditions, H2O2 was determined over the range of 1.0 × 10-10 to 1.0 × 10-8 mol L-1 with a detection limit of (3σ) of 4.1 × 10-11 mol L-1. The relative standard deviation (RSD) was 3.2% for 5 nmol L-1 H2O2 (n = 7). The proposed method offers the advantages of ultra-sensitivity, selectivity, simplicity and rapidity for H2O2 determination. It was successfully applied to directly determine trace amounts of H2O2 (nmol L-1) in human's EBC of both rheum and healthy volunteers. A statistically significant difference was found between patients with rheum (n = 11) and control subjects without rheum (n = 11).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 24 (2), 201-205, 2008
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233705344
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- NII論文ID
- 130004441601
- 10021098293
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 9362776
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- Web Site
- http://id.ndl.go.jp/bib/9362776
- https://ndlsearch.ndl.go.jp/books/R000000004-I9362776
- https://link.springer.com/content/pdf/10.2116/analsci.24.201.pdf
- https://link.springer.com/article/10.2116/analsci.24.201/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/24/2/24_2_201/_pdf
- https://search.jamas.or.jp/link/ui/2008187568
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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