Determination of Superoxide Scavenging Activity of a Sample Containing Xanthine Oxidase Inhibitor by ESR Spin Trapping.

KOIDE Tomoko, NODA Hiroyuki, LIU Wenwei, OGATA Tateaki, KAMADA Hitoshi

doi:10.2116/analsci.16.1029

Determination of Superoxide Scavenging Activity of a Sample Containing Xanthine Oxidase Inhibitor by ESR Spin Trapping.

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KOIDE Tomoko

Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry
NODA Hiroyuki

Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry
LIU Wenwei

Graduate School of Science and Engineering, Yamagata University
OGATA Tateaki

Graduate School of Science and Engineering, Yamagata University
KAMADA Hitoshi

Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry

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抄録

The objective of this research was to determine the true superoxide (O₂^-) scavenging activity of antioxidants in a sample containing xanthine oxidase (XOD) inhibitor. A previous method involving electron spin resonance (ESR) spin-trapping has generally used 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) as a spin trap and the O₂^- generation system of hypoxanthine (HPX)-xanthine oxidase (XOD). However, this method has a problem in that the ESR signal intensity of the DMPO spin adduct of O₂^- (DMPO-OOH) is decreased not only by the reaction of a sample with O₂^-, but also by the inhibition of XOD. Therefore, the ESR spin-trapping method combined with the SOD addition method was developed as a new experimental technique in this study. The results indicate that the O₂^- scavenging activity of a sample containing allopurinol as an XOD inhibitor could be determined by using this method.