Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label

YOSHIKAWA Kenji, YUAN Jingli, MATSUMOTO Kazuko, KIMURA Hiroko

doi:10.2116/analsci.15.121

Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label

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YOSHIKAWA Kenji

Department of Chemistry, Waseda University
YUAN Jingli

Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
MATSUMOTO Kazuko

Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
KIMURA Hiroko

Japan Science and Technology Corporation (JST) Department of Forensic Medicine, Juntendo University School of Medicine

書誌事項

タイトル別名

Time-Resolved Fluorometric Detection of DNA Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.
Time-Resolved Fluorometric Detection of DNA Using a Tetradentate ベーターDiketonate Europium Chelate as a Label

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抄録

A tetradentate β-diketonate europium chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chloro-sulfo-o-terphenyl(BHHCT)-Eu³⁺, was labeled to a streptavidin-bovine serum albumin conjugate for time-resolved fluorometric detection of λDNA by hybridization with a biotinylated probe DNA in a microtiter well. The method gave a detection limit of 23 pg/well (solid-phase measurement) and 4 pg/well (solution-phase measurement) for specific DNA.