Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe

SONG Gongwu, LI Ling, LIU Liming, FANG Guangrong, LU Shaofang, HE Zhike, ZENG Yun’e

doi:10.2116/analsci.18.757

Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe

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SONG Gongwu

Analysis and Testing Center Hubei University
LI Ling

Analysis and Testing Center Hubei University
LIU Liming

Analysis and Testing Center Hubei University
FANG Guangrong

Analysis and Testing Center Hubei University
LU Shaofang

College of Chemistry, Wuhan University
HE Zhike

College of Chemistry, Wuhan University
ZENG Yun’e

College of Chemistry, Wuhan University

書誌事項

タイトル別名

Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)<sub>2</sub>PIP(V) as a Nucleic Acid Probe

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抄録

A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)₂PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)₂PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.