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Optical Characterization of the Antigen–Antibody Thin Layer Using the Whispering Gallery Mode
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- TAJIRI Takeshi
- Industrial Technology Center of Nagasaki Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima
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- MATSUMOTO Shuzo
- Industrial Technology Center of Nagasaki
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- IMATO Toshihiko
- Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
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- OKAMOTO Toshihiro
- Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima
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- HARAGUCHI Masanobu
- Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima
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Description
We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen–antibody layer from the shift of the WGM spectra peak.
Journal
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- Analytical Sciences
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Analytical Sciences 30 (8), 799-804, 2014
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679236571264
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- NII Article ID
- 130004677854
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- NII Book ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2M%2Fgs1OrsA%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 025633208
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- PubMed
- 25109641
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed