PNA-tagged Peptide Microarrays for Ratiometric Activity Detection of Cellular Protein Kinases
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- IKEDA Hiromu
- Department of Applied Chemistry, Kyushu University
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- YAYAMA Yoshihiro
- Department of Applied Chemistry, Kyushu University
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- HATA Akito
- Department of Applied Chemistry, Kyushu University
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- KAMIMOTO Jumpei
- Department of Applied Chemistry, Kyushu University
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- YAMAMOTO Tatsuhiro
- Institute of System, Information Technologies and Nanotechnologies
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- MORI Takeshi
- Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
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- KATAYAMA Yoshiki
- Department of Applied Chemistry, Kyushu University Center for Future Chemistry, Kyushu University
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抄録
Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 30 (6), 631-635, 2014
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679237212800
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- NII論文ID
- 130004442298
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2cfhs1Cisg%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 025501959
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- PubMed
- 24919666
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- Web Site
- http://id.ndl.go.jp/bib/025501959
- https://ndlsearch.ndl.go.jp/books/R000000004-I025501959
- https://link.springer.com/content/pdf/10.2116/analsci.30.631.pdf
- https://link.springer.com/article/10.2116/analsci.30.631/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/6/30_631/_pdf
- https://search.jamas.or.jp/link/ui/2015097403
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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