Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction

DOI PDF Web Site Web Site Web Site View 1 Remaining Hide 18 Citations 8 References

Search this article

Description

We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new method could avoid hindering fluorescence detection, even if the templates were used directly. Saliva was needed to be put on water-soluble paper and dried, and hairs were cut to be about 10 mm long. This method could reduce both the time and effort involved, and also the risk of contamination. It should prove to be very valuable for genetic diagnoses in various fields.

Journal

  • Analytical Sciences

    Analytical Sciences 30 (3), 427-429, 2014

    The Japan Society for Analytical Chemistry

Citations (18)*help

See more

References(8)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top