Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
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- HAYASHIDA Mariko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- OTA Tomoko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- ISHII Minori
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- IWAO-KOIZUMI Kyoko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- MURATA Shigenori
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- KINOSHITA Kenji
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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説明
We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new method could avoid hindering fluorescence detection, even if the templates were used directly. Saliva was needed to be put on water-soluble paper and dried, and hairs were cut to be about 10 mm long. This method could reduce both the time and effort involved, and also the risk of contamination. It should prove to be very valuable for genetic diagnoses in various fields.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 30 (3), 427-429, 2014
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679237332352
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- NII論文ID
- 130004827428
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2crhslOgtg%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 025345730
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- PubMed
- 24614740
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- Web Site
- http://id.ndl.go.jp/bib/025345730
- https://ndlsearch.ndl.go.jp/books/R000000004-I025345730
- https://link.springer.com/content/pdf/10.2116/analsci.30.427.pdf
- https://link.springer.com/article/10.2116/analsci.30.427/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/3/30_427/_pdf
- https://search.jamas.or.jp/link/ui/2014236669
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
