Analysis of Major Amino Acids in Green Tea by High-performance Liquid Chromatography Coupled with OPA Precolumn Derivatization

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  • GOTO Tetsuhisa
    National Research Insitute of Vegetables, Ornamental Plants and Tea
  • HORIE Hideki
    National Research Insitute of Vegetables, Ornamental Plants and Tea
  • MUAKAI Toshihiro
    National Research Insitute of Vegetables, Ornamental Plants and Tea

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  • 緑茶中の主要アミノ酸のOPAによるプレカラム誘導体化高速液体クロマトグラフィーによる分析
  • リョクチャチュウ ノ シュヨウ アミノサン ノ OPA ニ ヨル プレカラム

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Seven major amino acids and γ-amino butyric acid in green tea were analyzed within 25 minutes by using reversed phase high performance liquid chromatography coupled with precolumn derivatization by o-phthalaldehyde. Glycylglycine was used as an internal standard. To separate amino acids, a multi step linear gradient of the concentration of acetonitrile in citrate buffer was used. Average coefficient of variations of the eight amino acids were less than 0.5% for the retension time and about 1.1% for the quantity.

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