Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin

DOI Web Site 被引用文献22件 参考文献27件
  • Yamamura Masaomi
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Hattori Takefumi
    Institute of Socio-Arts and Sciences, The University of Tokushima
  • Suzuki Shiro
    Research Institute for Sustainable Humanosphere, Kyoto University
  • Shibata Daisuke
    Kazusa DNA Research Institute
  • Umezawa Toshiaki
    Research Institute for Sustainable Humanosphere, Kyoto University Institute of Sustainability Science, Kyoto University

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タイトル別名
  • Microscale thioacidolysis method for the rapid analysis of β-<i>O</i>-4 substructures in lignin
  • Microscale thioacidolysis method for the rapid analysis of ^|^#946;-O-4 substructures in lignin

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Thioacidolysis is a method to detect the β-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.

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