Double-stranded DNA introduction into intact plants using peptide–DNA complexes

DOI NDLデジタルコレクション Web Site Web Site 被引用文献9件 参考文献24件 オープンアクセス
  • Lakshmanan Manoj
    Enzyme Research Team, Biomass Engineering Program Cooperative Division, RIKEN Center for Sustainable Resource Science Ecobiomaterial Research Laboratory, School of Biological Science, Universiti Sains Malaysia
  • Yoshizumi Takeshi
    Institute for Advanced Biosciences, Keio University
  • Kodama Yutaka
    Center for Bioscience Research and Education, Utsunomiya University
  • Numata Keiji
    Enzyme Research Team, Biomass Engineering Program Cooperative Division, RIKEN Center for Sustainable Resource Science
  • Sudesh Kumar
    Ecobiomaterial Research Laboratory, School of Biological Science, Universiti Sains Malaysia

書誌事項

タイトル別名
  • Double-stranded DNA introduction into intact plants using peptide–DNA complexes
公開日
2015
資源種別
journal article
DOI
  • 10.5511/plantbiotechnology.14.1210b
公開者
日本植物バイオテクノロジー学会

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説明

Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact Nicotiana benthamiana leaves by using an ionic complex of a fusion peptide comprising (KH)9 and Bp100 with dsDNA encoding Renilla luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants.

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