Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an <i>Agrobacterium</i>-mediated RNAi technique
-
- Miyawaki Katsuyuki
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
-
- Fukuoka Sachi
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
-
- Kadomura Yasuko
- Department of Nutrition, Faculty of Medicine, The University of Tokushima
-
- Hamaoka Hirokazu
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
-
- Mito Taro
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
-
- Ohuchi Hideyo
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
-
- Schwab Wilfried
- Biotechnology of Natural Products, Technical University München
-
- Noji Sumihare
- Department of Life Systems, Institute of Technology and Science, The University of Tokushima
Bibliographic Information
- Other Title
-
- Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique
- 10.5511/plantbiotechnology.12.406a
Search this article
Description
Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an Agrobacterium-mediated RNAi (AmRNAi) technique in post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of Fa′chalcone synthase (Fa′CHS), Fa′chalcone isomerase (Fa′CHI), and Fa′flavonoid 3′-hydroxylase orthologues (Fa′F3′H) in post-harvest fruit. Fa′CHS and Fa′F3′H function was subsequently examined by performing AmRNAi with post-harvest fruit. Although reduction of light-induced Fa′F3′H expression by AmRNAi resulted in no significant change in anthocyanin content, reduction of Fa′CHS significantly decreased anthocyanin levels, and up-regulated Fa′F3′H levels. Our results are consistent with previous data indicating that while CHS is required for anthocyanin accumulation during late stage strawberry fruit maturation, Fa′F3′H is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit.
Journal
-
- Plant Biotechnology
-
Plant Biotechnology 29 (3), 271-277, 2012
Japanese Society for Plant Biotechnology
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679303628160
-
- NII Article ID
- 10030804744
- 130001883169
-
- NII Book ID
- AA11250821
-
- COI
- 1:CAS:528:DC%2BC38Xht1Chtb3M
-
- ISSN
- 13476114
- 13424580
- 08496757
-
- NDL BIB ID
- 023784456
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed