-
- NAKANO Yoshihiro
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
- OKAWA Satoshi
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
- YAMAUCHI Takayoshi
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
- KOIZUMI Yukio
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
- SEKIYA Jiro
- Plant Nutrition Laboratory, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
書誌事項
- タイトル別名
-
- Occurrence of Two Forms of .GAMMA.-GIutamyltransferases in Radish Plant
- Occurrence of Two Forms of ガンマ Glutamyltransferases in Radish Plant
この論文をさがす
抄録
In radish (Raphanus sativus L.) cotyledons, γ-glutamyltransferase (GGT, EC 2.3.2.2) activity was detected in both fractions extracted with grinding buffer alone (low ionic strength buffer) and the same buffer supplemented with 1 M NaCl (high ionic strength buffer). The former was referred to as soluble GGT and the latter, as bound GGT. Bound GGT was not solubilized by 1% Triton X-100. The activities of both soluble and bound GGTs were inhibited by acivicin, an inhibitor of mammalian and Escherichia coli GGTs. The activity of GGT extracted with the high ionic strength buffer disappeared when protoplasts were prepared, indicating the localization of bound GGT to cell walls. These results strongly indicate the occurrence of two forms of GGTs, soluble and bound ones. The two forms of GGTs were also found in Arabidopsis thaliana, cabbage, pea, soybean, maize and rice among the eight plants tested, in addition to radish.
収録刊行物
-
- Plant Biotechnology
-
Plant Biotechnology 21 (3), 243-246, 2004
日本植物バイオテクノロジー学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679304040320
-
- NII論文ID
- 10013522948
-
- NII書誌ID
- AA11250821
-
- COI
- 1:CAS:528:DC%2BD2cXptVKhuro%3D
-
- ISSN
- 13476114
- 13424580
-
- NDL書誌ID
- 7066761
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可