Establishment of <i>Agrobacterium</i>-mediated genetic transformation system in <i>Dahlia</i>
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- Otani Yuko
- Graduate School of Horticulture, Chiba University
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- Chin Dong Poh
- Graduate School of Horticulture, Chiba University
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- Mii Masahiro
- Graduate School of Horticulture, Chiba University
Bibliographic Information
- Other Title
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- Establishment of Agrobacterium-mediated genetic transformation system in Dahlia
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Abstract
An efficient system for Agrobacterium-mediated transformation was established in dahlia (Dahlia×pinnata) ‘Yamatohime’. Mass of shoot primordia (MSP) induced on MS medium supplemented with 10 mg l−1 TDZ were inoculated with Agrobacterium tumefaciens strain EHA101 (pIG121-Hm) harboring both β-glucuronidase (GUS) and hygromycin resistant genes. After 2 days of co-cultivation, the MSP were transferred to a selection medium containing hygromycin with meropenem for bacterial elimination. Shoots were successfully regenerated from survived MSP on hormone-free medium without hygromycin and they rooted on hormone-free medium containing hygromycin. The hygromycin-resistant plants thus obtained showed histochemical blue staining for GUS. Transformation of plants was confirmed by PCR and Southern blot analyses. Transgenic ‘Kokucho’ were also produced by using the same transformation procedure, suggesting wide applicability of this Agrobacterium-mediated transformation procedure for other dahlia cultivars.
Journal
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- Plant Biotechnology
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Plant Biotechnology 30 (2), 135-139, 2013
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679304255232
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- NII Article ID
- 10031184117
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC3sXhtlCqsL3I
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 024676569
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed