Isolation and expression analysis of candidate genes related to <italic>Ralstonia</italic> <italic>solanacearum</italic> – tobacco interaction
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- Kiba Akinori
- Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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- Maimbo Milimo
- Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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- Kanda Ayami
- Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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- Tomiyama Hiromi
- Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
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- Ohnishi Kouhei
- Research institute of Molecular Genetic, Kochi University
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- Hikichi Yusufumi
- Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
書誌事項
- タイトル別名
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- Isolation and expression analysis of candidate genes related to Ralstonia solanacearum-tobacco interaction
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Ralstonia solanacearum is a causal agent of bacterial wilt, and the bacterium normally infects through wounds in roots. Since it is not possible to equally and simultaneously inoculate the host plants with bacteria via root inoculation, it has proven difficult to elucidate the molecular events in plants infected with R. solanacearum. To improve the efficiency of inoculation, we inoculated tobacco plants with R. solanacearum by leaf-infiltration. Differential display was carried out to isolate fragments of genes that are regulated in tobacco by virulent strains of R. solanacearum OE1-1 and by the avirulent mutant of R. solanacearum, 31b, which is mutated in the type III secreted PopA protein by transposon insertion. Nineteen R. solanacearum-responsive genes (RsRGs) were successfully isolated using equalized cDNA libraries constructed with water-, R. solanacearum OE1-1- and 31b-infiltrated tobacco leaves. From Northern blot analysis, RsRGs were divided into 3 groups; 1) responsive to both virulent and avirulent bacteria (8 RsRGs), 2) responsive to avirulent bacteria (3 RsRGs), and 3) responsive to virulent bacteria (3 RsRGs). These results suggest that the cDNA pool and method presented in this study provide a valuable resource for functional genomic analysis of R. solanacearum-plant interactions.
収録刊行物
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- Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ
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Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 24 (4), 409-416, 2007
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679304464128
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- NII論文ID
- 10021911916
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2sXhtF2rtrrJ
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 8904962
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可