Molecular cloning of cDNA for a novel pea Dof protein, PsDof1, and its DNA binding activity to the promoter of PsDofl gene

DOI Web Site 被引用文献4件 参考文献38件
  • SEKI Hikaru
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • NAKAMURA Naoki
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • MARUTANI Mizuri
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • OKABE Takeharu
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • SANEMATSU Shiroh
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • INAGAKI Yoshishige
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • TOYODA Kazuhiro
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • SHIRAISHI Tomonori
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • YAMADA Tetsuji
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
  • ICHINOSE Yuki
    Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University

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  • Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene.

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A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn2+ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

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