ホオノキの茎頂培養による多芽体誘導

DOI Web Site 被引用文献3件 参考文献12件 オープンアクセス

書誌事項

タイトル別名
  • Induction of Multiple shoots by Shoot Apex Culture in Magnolia obovata Thunb.
  • ホオノキの茎頂培養による多芽体誘導〔英文〕
  • ホオノキ ノ ケイチョウ バイヨウ ニ ヨル タ ガタイ ユウドウ エイブン

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説明

Shoot apex of Magnolia obovata Thunb. was cultured on the modified Murashige and Skoog's (MS) medium supplemented with the combinations of IBA and BAP or NAA and BAP in various concentrations. Multiple shoots were induced successfully on the half strength MS reducing nitrate to one fourth (1/4N-1/2MS) medium containing 4μM IBA and 5μM BAP after 40 days of culture. The multiple shoots obtained were divided into small nodal explants and further subcultured on fresh medium. Many axillary buds were induced from the explant after 20 days of culture, and they succeedingly grew actively and developed multiple shoots. Culturing the axillary bud together with the stem tissue was effective for facilitating the induction of multiple shoots.

収録刊行物

  • 植物組織培養

    植物組織培養 12 (1), 34-40, 1995

    日本植物細胞分子生物学会

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