Visualization of Sialic Acid Produced on Bacterial Cell Surfaces by Lectin Staining

DOI Web Site Web Site PubMed 3 Citations 32 References Open Access
  • Kajiwara Hitomi
    Glycotechnology Business Unit, Japan Tobacco Inc.
  • Toda Munetoyo
    Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
  • Mine Toshiki
    Glycotechnology Business Unit, Japan Tobacco Inc.
  • Nakada Hiroshi
    Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
  • Wariishi Hiroyuki
    Department of Forest and Forest Products Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyusyu University
  • Yamamoto Takeshi
    Glycotechnology Business Unit, Japan Tobacco Inc.

Search this article

Description

Oligosaccharides containing N-acetylneuraminic acid on the cell surface of some pathogenic bacteria are important for host-microbe interactions. N-acetylneuraminic acid (Neu5Ac) plays a major role in the pathogenicity of bacterial pathogens. For example, cell surface sialyloligosaccharide moieties of the human pathogen Haemophilus influenzae are involved in virulence and adhesion to host cells. In this study, we have established a method of visualizing Neu5Ac linked to a glycoconjugate on the bacterial cell surface based on lectin staining. Photobacterium damselae strain JT0160, known to produce a-2,6-sialyltransferase, was revealed to possess Neu5Ac by HPLC. Using the strain, a strong Sambucus sieboldiana lectin-binding signal was detected. The bacteria producing α-2,6-sialyltransferases could be divided into two groups: those with a lot of α-2,6-linked Neu5Ac on the cell surface and those with a little. In the present study, we developed a useful method for evaluating the relationship between Neu5Ac expression on the cell surface and the degree of virulence of marine bacteria.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 25 (3), 152-155, 2010

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Citations (3)*help

See more

References(32)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top