細管式等速電気泳動法におけるカウンター・フローの効果

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書誌事項

タイトル別名
  • 細管式等連電気泳動法におけるカウンター・フローの効果
  • Electrolyte counterflow in isotachophoresis
  • サイカンシキ トウソク デンキ エイドウホウ ニ オケル カウンター フロー

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説明

A syring-type counterflow device for isotachophoresis has been developed to separate ions that differ only little in mobility. The device was installed in a Shimadzu Isotachophoretic Analyzer IP-1, in order to study the effects of the counterflow on the separation efficiency. The migration current was set to 100 μA; the capillary tube was 50 cm long; the sample was a mixture of aqueous solutions of 0.01 M sodium oxalate, 0.01M sodium formate and 0.01M sodium citrate. The rate of counterflow was varied in 6 steps of 6, 12, 25, 50, 100 and 200 μl/h, by changing the plunger speed. Thus, separation efficiency has been studied as a function of sample volume and counterflow rate. A mixed zone of formate and citrate was observed for a sample volume above 8 μl when counterflow was not applied. This mixed zone was eliminated when counterflow was applied at 200μl/h for 15 min. The effective length of the capillary tube can be increased by 40% by applying a counterflow.

収録刊行物

  • 分析化学

    分析化学 24 (11), 728-729, 1975

    公益社団法人 日本分析化学会

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